新的更简单和具有成本效益的法医测试有助于识别触摸DNA

2024年3月14日,星期四

实验室工作人员用棉签擦拭枪把

Samantha McCrane of UNH’s Forensic Anthropology Identification and Recovery (FAIR) Lab swabs a gun grip to look for trace DNA during 研究 trials with a less expensive and easy to use DNA test.

N达勒姆.H. ——像《永利app新版本官网地址》和《永利app新版本官网地址》这样的电视剧让刑事调查看起来很容易. 在现实生活中, DNA测试具有挑战性,需要昂贵的设备, special facilities and extensive training to identify DNA from a crime scene and determine which belongs to a potential suspect and which may have been transferred from someone who was never there. Research from the 永利app新版本官网地址 has found a less expensive and easier to use test to learn more about forensic touch DNA. This research has important implications for forensic investigations and being able to identify DNA from a primary contact—someone who may have committed the crime—as well as secondary DNA that was inadvertently and indirectly transferred through touch.

“So-called ‘touch DNA’ is a form of trace DNA that is deposited when a person touches something and leaves behind their skin cells, 汗液或其他含有它们DNA的液体,萨曼莎·麦克兰说, a lecturer in anthropology and co-director of UNH’s Forensic Anthropology Identification and Recovery (FAIR) Lab. “而接触DNA通常是直接接触的结果, 我们称之为初级转移, 它也可以在物体表面或个体之间间接传递, 留下了二级甚至三级DNA.”

在他们的 研究, 最近发表在《永利app新版本官网地址》上, researchers developed an innovative test that uses a more accessible and affordable sequence method, 称为qPCR. 来测试他们的协议, 他们将男性和女性志愿者配对,并保持简单, 只看一个标记来确定DNA的性别. 在审判中, they first had a male participant hold a gun grip for 30 seconds before placing it down on a sterilized table. 然后, a female picked up the same gun grip and held it for 30 seconds and followed that by grasping a coffee cup for 30 seconds. 之后,枪柄、咖啡杯和女性的手都被提取了DNA.

的 findings with the new method found male and female DNA on the gun grip in 71% of the trials indicating primary transfer since both participants directly touched the gun grip. Male DNA was found on the female's hand in 50% of the trials representing secondary transfer since the DNA was transferred indirectly from the gun grip. 咖啡杯上有27%的男性DNA被记录为第三代, 或者第三层, transfer since the DNA was indirectly transferred from the gun grip to the female's hand and finally to the coffee mug.

“的 challenge with transfer DNA is that it opens up the dangerous possibility of DNA ending up on items or victims at a crime scene that a person may not have touched” said McCrane. “这种情况已经发生在多起案件中, 导致无辜的人因他们没有犯下的罪行而受到指控.”

的 研究 also looked at the potential effects of age, ethnicity and skin conditions on DNA transfer. Ethnicity and age did not appear to affect touch DNA deposits and the small sample of those with sloughing skin conditions, 像湿疹, 没有显示出与原代DNA转移有任何显著关联.

Researchers say even DNA experts cannot distinguish between different types of DNA transfer and this understudied field lacks enough data to fully understand which variables affect direct versus indirect DNA transfer and how often it happens. 的se new 研究 results contribute to a better understanding of the conditions under which secondary and tertiary DNA transfer occurs and researchers are hopeful this new inexpensive protocol could lead to more research allowing for greater sample sizes and replication runs.

Co-author on the 研究 was Connie Mulligan, professor of anthropology at the University of Florida.

永利app新版本官网地址 激励创新,改变我们国家、民族和世界的生活. 超过16个,000 students from 49 states and 82 countries engage with an award-winning faculty in top-ranked programs in business, 工程, 法律, 卫生和公共服务, 文科和理科横跨200多个学习项目. 卡内基R1分类机构, 联合国大学与美国宇航局合作, 美国国家海洋和大气管理局, NSF, 和国家卫生研究院, and received over $210 million in competitive external funding in FY23 to further explore and define the frontiers of land, 海洋与太空.

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链接: http://www.主要study.edu/主要studytoday/sites/default/files/lab2.jpg

Caption: Samantha McCrane of UNH’s Forensic Anthropology Identification and Recovery (FAIR) Lab swabs a gun grip to look for trace DNA during 研究 trials with a less expensive and easy to use DNA test.

图片来源:UNH

 

链接: http://www.主要study.edu/主要studytoday/sites/default/files/lab1.jpg

Caption: 的 handle of a gun is swabbed to search for trace DNA using a new less expensive and easy to use DNA test.

图片来源:UNH